Plasmid_Backbone

Part:BBa_K4974015

Designed by: Benjamin Li   Group: iGEM23_NYCEmpireState   (2023-10-07)


pET-28a(+)-FGF2-G3

We purchased this from Addgene (https://www.addgene.org/135521/).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 656
    Illegal XbaI site found at 47
    Illegal PstI site found at 373
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 656
    Illegal NheI site found at 151
    Illegal PstI site found at 373
    Illegal NotI site found at 681
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 656
    Illegal BglII site found at 5812
    Illegal BamHI site found at 184
    Illegal XhoI site found at 690
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 656
    Illegal XbaI site found at 47
    Illegal PstI site found at 373
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 656
    Illegal XbaI site found at 47
    Illegal PstI site found at 373
    Illegal NgoMIV site found at 989
    Illegal NgoMIV site found at 4032
    Illegal NgoMIV site found at 4192
    Illegal NgoMIV site found at 5780
    Illegal AgeI site found at 604
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 3114


[edit]
Categories
Parameters
None